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目的:观察长期、大量给予益母草醇提取物导致大鼠肾毒性机制与氧化损伤的相关性。方法:按90天毒性实验法,检测血中丙二醛(MDA)、总巯基(-SH)、还原型谷胱甘肽(GSH)含量以及超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)的活性。结果:益母草醇提取物可导致血中总-SH、MDA含量增加,GSH含量降低;SOD、GSH-Px活性下降;上述变化随剂量的增加而逐渐加重,与空白对照组相比有明显差异。结论:益母草醇提取物导致大鼠肾毒性损伤途径与引起机体的氧化应激诱导肾细胞脂质过氧化作用增强有关,也与机体氧化应激后诱导脂质过氧化和组织内活性分子-SH损耗而造成肾组织损伤有关。
OBJECTIVE: To observe the correlation between long-term and large-dose administration of Leonurus amylol extract to oxidative damage and the mechanism of nephrotoxicity in rats. Methods: The levels of malondialdehyde (MDA), total thiol (-SH), glutathione (GSH) and superoxide dismutase (SOD), glutathione Peroxidase (GSH-Px) activity. Results: Leonuriella extract could result in the increase of blood total-SH, MDA content, GSH content and the activity of SOD, GSH-Px. The above changes gradually aggravated with the increase of dose, which were significantly different from the blank control group. CONCLUSION: The toxic effects of Leonurus extract on renal nephrotoxicity in rats are related to the enhancement of lipid peroxidation in renal cells induced by oxidative stress. The oxidative stress induced by oxidative stress induces lipid peroxidation and activation of the active molecule -SH Loss caused by renal tissue damage.