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目的采用聚合酶链式反应(PCR)和脉冲场电泳(PFGE)在不同角度和层次的研究和探讨,为明确食物中毒诊断提供依据,并为不同时期、不同地区、不同检测要求的疾病预防控制提供新的手段和思路。方法用各种采集样品进行PCR检测,并且用检测到的肠毒素进行同源性调查研究。结果 3例食物中毒事件中分离到金黄色葡萄球菌菌株中检测出肠毒素及其相应的编码基因,并用PFGE证实了3株菌属于同一克隆,但本次的金黄色葡萄球菌经比较与另一次导致食物中毒事件中分离的金黄色葡萄球菌不具有同源性。结论 PCR快速有效的鉴定病原菌,PFGE能准确地追溯其同源性,为食物中毒病原菌鉴定提供快速而可靠的方法。
OBJECTIVE: To study and explore the different angles and levels of polymerase chain reaction (PCR) and pulsed-field electrophoresis (PFGE) to provide evidence for the diagnosis of food poisoning and to control the disease in different periods, different regions and different testing requirements Provide new means and ideas. Methods Various samples were collected for PCR detection and homology investigation with the detected enterotoxins. Results Staphylococcus aureus isolates were isolated from three kinds of food poisoning cases and their corresponding coding genes were detected. PFGE confirmed that the three strains belonged to the same clone, but Staphylococcus aureus was compared with another Staphylococcus aureus isolated in a food poisoning event does not have homology. Conclusion PCR is a rapid and effective method for the identification of pathogenic bacteria. PFGE can accurately trace its homology and provide a fast and reliable method for the identification of pathogenic bacteria.