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本文应用组织化学、乙醛酸诱发荧光等法显示肾上腺素能(NA)神经;胆碱酯酶(AchE)直接染色法显示胆碱能神经;免疫组织化学 PAP 法显示血管活性肠肽(VIP)与神经肽 Y(NPY)免疫反应神经。对大鼠输精管的观察表明,VIP 免疫反应性神经与胆碱能神经分布基本一致,主要位于固有层内,而 NPY 免疫反应神经与肾上腺素能神经分布基本一致,主要位于输精管肌层。于第1、2、6、7天腹腔注射6-OHDA 4次,第8天处死动物进行观察,此时输精管肌层中的肾上腺素能神经几乎全部消失,其减少程度与 NPY 免疫反应神经基本一致;而 VIP 与胆碱能神经未见变化。免疫电镜 PAP 包埋前染色法显示,VIP 免疫复合物沉积于含直径40～55nm 的清亮小囊泡的膨体中,有的膨体除清亮小囊泡外,还含有少量的大颗粒囊泡(直径100～144nm),NPY 免疫复合物主要沉积于大颗粒囊泡中,偶见于直径40～55nm 的小颗粒囊泡中。以上实验结果提示,在大鼠输精管,VIP 可能与 ACh 共存,NPY 可能与去甲肾上腺素共存。
In this study, adrenergic (NA) nerves were visualized by histochemistry and glycolate-induced fluorescence. Cholinergic nerves were visualized by direct cholinesterase (AchE) staining. Vasoactive intestinal peptide (VIP) Immunoreactive nerve with neuropeptide Y (NPY). Observations on the vas deferens in rats showed that VIP immunoreactive nerve and cholinergic nerve distribution were basically the same, mainly located in the lamina propria, while the NPY immunoreactive nerve and adrenergic nerve distribution were basically the same, mainly in the vas deferens muscle layer. On the 1st, 2nd, 6th and 7th day, 6-OHDA was intraperitoneally injected for 4 times and the animals were sacrificed on the 8th day. At this time, almost all the adrenergic nerves in the vas deferens muscle layer disappeared. The degree of reduction was similar to that of the NPY immunoreactive nerve Consistent; VIP and cholinergic nerve no change. Immunoelectron microscopy PAP preincubation staining showed that the VIP immune complexes were deposited on the bulky vesicles with diameters of 40-55 nm. Some bulks, in addition to the clear vesicles, contained small amounts of large-sized vesicles (Diameter of 100 ~ 144nm), NPY immune complexes mainly deposited in large particle vesicles, occasionally in small particle vesicles 40 ~ 55nm in diameter. The above experimental results suggest that vas deferens in rats, VIP may coexist with ACh, NPY may coexist with norepinephrine.