论文部分内容阅读
目的观察槲皮素对HepG2细胞增殖和凋亡的影响并探讨其机制。方法人肝癌HepG2细胞系,分为6组,A～E组加入终浓度分别为12.5,25.0,50.0,100.0,200.0μmol/L槲皮素,F组为空白对照组,加入等量营养液。采用MTT法及流式细胞检测技术检测各组人肝癌HepG2细胞对槲皮素的敏感性,应用免疫细胞化学技术检测细胞中Bcl-2及Bax蛋白的表达情况。结果槲皮素对肝癌HepG2细胞的增殖呈浓度依赖性的抑制作用,作用48h后,A～E组抑制率与凋亡率高于F组,差异均有统计学意义(P<0.05);槲皮素作用HepG2细胞24h后,D组G1期细胞比率高于F组,差异有统计学意义(P<0.05),S和M期细胞比率差异无统计学意义(P>0.05);作用48h后,D组Bcl-2和Bax表达灰度值与F组比较,差异有统计学意义(P<0.05)。结论槲皮素对肝癌HepG2细胞具有生长抑制作用,并通过激活线粒体凋亡途径来促进细胞凋亡。
Objective To observe the effect of quercetin on the proliferation and apoptosis of HepG2 cells and to explore its mechanism. Methods HepG2 human hepatocellular carcinoma cell lines were divided into 6 groups. The final concentrations of A, E and E were 12.5, 25.0, 50.0, 100.0 and 200.0 μmol / L, respectively. The F group was blank control group, and the same amount of nutrient solution was added. MTT assay and flow cytometry were used to detect the sensitivity of HepG2 cells to quercetin in each group. The expression of Bcl-2 and Bax proteins was detected by immunocytochemistry. Results Quercetin inhibited the proliferation of HepG2 cells in a concentration-dependent manner. After 48 hours, the inhibitory rates and apoptotic rates of A to E groups were higher than those of F group (P <0.05) After 24h of HepG2 cells treated with dexamethasone for 24 hours, the percentage of cells in G1 phase in group D was higher than that in group F (P <0.05). There was no significant difference in the percentage of cells in S and M phase (P> 0.05) The gray value of Bcl-2 and Bax expression in group D was significantly higher than that in group F (P <0.05). Conclusion Quercetin can inhibit the growth of HepG2 hepatocarcinoma cell line and promote cell apoptosis by activating the mitochondrial apoptosis pathway.