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:【目的】探讨高温联用鬼臼噻吩甙(Vm26)诱导白血病细胞的凋亡及净化。【方法】13例急性白血病骨髓、15例正常骨髓和10例次HL60细胞株用形态学、DNA凝胶电泳、流式细胞仪及半固体集落培养技术,观察42℃60min高温或联用鬼臼噻吩甙(Vm26)诱导HL60细胞的凋亡其及体外净化白血病细胞的效果。【结果】①HL60细胞经42℃60min、42℃60min+85μmin/LVm26处理后48h流式细胞检测的凋亡细胞(subG1%)达高峰,分别为764%±78%、934%±36%,P<005。DNA凝胶电泳24～48h处理后出现典型的梯状图谱,而对于正常骨髓细胞经上述同样处理后subG1分别为186%±51%、401%±32%(与HL60细胞比较P<001)。②高温42℃60min联用Vm26体外净化白血病细胞集落(LCFU)、HL60的抑制率分别为992%±1.8%、100%。而正常粒巨噬细胞集落(GMCFU)的抑制率为495%±45%。【结论】高温42℃60min联用Vm26诱导HL60细胞的凋亡比对正常造血细胞的凋亡强,高温联用Vm26是一种较好的体外净化方法
: 【Objective】 To investigate the apoptosis and purification of leukemia cells induced by high temperature combined with tenufopidine (Vm26). 【Methods】 Thirteen cases of acute leukemia bone marrow, 15 normal bone marrow and 10 cases of secondary HL60 cells were observed by morphology, DNA gel electrophoresis, flow cytometry and semi-solid colony culture. Apoptosis of HL60 cells induced by thymidine (Vm26) and its effect on purifying leukemic cells in vitro. 【Results】 (1) The apoptotic cells (subG1%) of HL60 cells detected by flow cytometry after 48 ℃ for 60min, 42 ℃ for 60min and 85μmin / LVm26 for 48h reached 764% ± 78%, 934% ± 36%, P <005. DNA ladder electrophoresis after 24 ~ 48h treatment showed a typical ladder pattern, and for normal bone marrow cells after the same treatment subG1 were 186% ± 51%, 401% ± 32% (compared with HL60 cells P <001). ② The inhibition rates of LCFU and HL60 in combination with Vm26 at 42 ℃ for 60min were 992% ± 1.8% and 100%, respectively. The inhibition rate of normal granulocyte colony (GMCFU) was 495% ± 45%. 【Conclusion】 Apoptosis of HL60 cells induced by high temperature at 60 ℃ for 60min combined with Vm26 is stronger than that of normal hematopoietic cells, and high temperature combined with Vm26 is a better method for in vitro purification