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目的建立RP-HPLC测定忍冬藤中绿原酸、咖啡酸、当药苷和马钱子苷含量的方法,并以此对忍冬藤进行质量控制。方法色谱柱为Aglient Extend-C18(4.6 mm×250 mm,5μm);流动相为甲醇-0.4%醋酸水溶液(26∶74);流速1.0 mL-min-1,检测波长240 nm。结果绿原酸、咖啡酸、当药苷和马钱子苷的线性范围和相关系数分别为23.5～470 mg-L-1,r=0.999 9;12~240 mg-L-1,r=0.999 7;10.5～210 mg-L-1,r=0.999 9;13~260 mg-L-1,r=0.999 8。平均回收率分别为97.7%(RSD=2.23%),96.6%(2.29%),95.0%(2.26%),103.3%(2.93%)。结论本方法简单、快速、可靠,可用于忍冬藤药材的质量控制。
OBJECTIVE To establish a RP-HPLC method for the determination of chlorogenic acid, caffeic acid, rhodopsin and strychnine in honeysuckle vinegar and to control its quality. Methods The column was Aglient Extend-C18 (4.6 mm × 250 mm, 5 μm). The mobile phase was methanol-0.4% acetic acid (26:74). The flow rate was 1.0 mL-min-1. Results The linear ranges and correlation coefficients of chlorogenic acid, caffeic acid, glycosides and strychnosin were 23.5-470 mg-L-1, r = 0.999 9, 12-240 mg-L-1, r = 0.999 7, 10.5-210 mg-L-1, r = 0.999 9, 13-260 mg-L-1, r = 0.999 8. The average recoveries were 97.7% (RSD = 2.23%), 96.6% (2.29%), 95.0% (2.26%) and 103.3% (2.93%), respectively. Conclusion The method is simple, rapid and reliable and can be used for the quality control of honeysuckle.