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目的探讨抗辐灵对微波辐射致大鼠睾丸组织脂质过氧化损伤的保护作用。方法 120只Wistar雄性大鼠,随机分为正常对照组、辐射对照组、阳性药对照组、0.75 g/(kg·d)抗辐灵组、1.5 g/(kg·d)抗辐灵组和3 g/(kg·d)抗辐灵组,30 m W/cm2微波全身辐射15 min,各组大鼠于辐射后当天开始灌胃给药,每天1次,连续14 d。分别于给药7 d(辐射后7 d)、停药后6 h(辐射后14 d)、停药后7 d(辐射后21 d)和停药后14 d(辐射后28 d)处死大鼠,光镜和电镜观察大鼠睾丸组织形态学和超微结构改变;酶标仪检测睾丸各组组织中活性氧(ROS)、脂质过氧化损伤指标(MDA)、抗氧化酶活性指标(SOD)。结果 30 m W/cm2微波辐射后,睾丸组织生精小管生精上皮疏松,生精细胞变性、坏死、脱落,精子明显减少;0.75 g/(kg·d)抗辐灵组与辐射对照组睾丸组织结构损伤特点、规律相似,仅程度较轻;1.5 g/(kg·d)抗辐灵组部分生精小管生精上皮疏松;3 g/(kg·d)抗辐灵组和阳性药对照组病变相似。30 m W/cm2微波辐射后睾丸组织中ROS、MDA含量升高(P<0.05),SOD活性下降(P<0.05);与辐射对照组相比,1.5和3 g/(kg·d)抗辐灵组于给药后ROS、MDA含量下降(P<0.05),SOD活性升高(P<0.05)。结论 30 m W/cm2微波辐射可引起大鼠睾丸结构损伤,氧化应激加强;抗辐灵具有拮抗微波辐射导致的大鼠睾丸组织结构损伤、抑制脂质过氧化、增强SOD活性作用,其中1.5和3g/(kg·d)抗辐灵组效果明显。
Objective To investigate the protective effect of Kang Ling on the lipid peroxidation of testis in rats induced by microwave radiation. Methods 120 Wistar male rats were randomly divided into normal control group, radiation control group, positive drug control group, 0.75 g / (kg · d) anti-Ling Ling group and 1.5 g / (kg · d) 3 g / (kg · d) radium-resistant group, 30 mW / cm2 microwave radiation for 15 min. Rats in each group began gavage on the day after radiation, once a day for 14 days. The rats were sacrificed at 7 days after radiation (7 days after radiation), 6 hours after radiation (14 days after radiation), 7 days after radiation (21 days after radiation) and 14 days after radiation (28 days after radiation) The morphological and ultrastructural changes of testis were observed under light, light and electron microscopes. The contents of reactive oxygen species (ROS), lipid peroxidation (MDA) and antioxidant enzyme activity SOD). Results After 30 mW / cm2 microwave irradiation, the spermatogenic epithelium of the testicular seminiferous tubules became loose and the spermatogenic cells became degenerated, necrotic and exfoliated, and the number of spermatozoa decreased significantly. In the test group of 0.75 g / (kg · d) The histological characteristics were similar with only a slight degree of damage. The germinal fine degeneration of some seminiferous tubules of 1.5 g / (kg · d) anti-Spangle group was loose; the control group of 3 g / (kg · d) Group lesions similar. The contents of ROS and MDA were increased (P <0.05) and the activity of SOD was decreased in the testis tissue after 30 mW / cm2 microwave radiation (P <0.05). Compared with the radiation control group, the levels of ROS and MDA The concentration of ROS and MDA in Radix-Ling group decreased (P <0.05) and SOD activity increased (P <0.05). Conclusion Microwave radiation at 30 mW / cm2 can induce the damage of testicular structure and oxidative stress in rats. Radix Rehmanniae can antagonize the damage of testicular tissue structure, inhibit the lipid peroxidation and enhance the activity of SOD induced by microwave radiation. 1.5 And 3g / (kg · d) anti-Ling group obvious effect.