A novel fluidized bed reactor was designed and installed for bioleaching in a semi continuous way, by which a process for bioleaching cyanidation of Jinya refractory gold arsenical concentrate was studied. The arsenic extraction rate reaches 82.5% after 4 day batch biooxidation of the concentrate under the optimized condition of pH 2.0, ferric ion concentration 6.5?g/L and pulp concentration 10%. And leached rate of gold in the following cyanidation is over 90%. The parameters of three series fluidized bed reactors exhibit stability during the semi continuous bioleaching of the concentrate. Arsenic in the concentrate can be got rid of 91% after 6 day leaching. Even after 4 days, 82% of arsenic extraction rate was still obtained. The recovery rates of gold are 92% and 87.5% respectively in cyaniding the above bioleached residues. The results will provide a base for further commercial production of gold development. A novel fluidized bed reactor was designed and installed for bioleaching in a semi continuous way, by which a process for bioleaching cyanidation of Jinya refractory gold arsenical concentrate was studied. The arsenic extraction rate reaches 82.5% after 4 day batch biooxidation of the concentrate under the optimized condition of pH 2.0, ferric ion concentration 6.5 μg / L and pulp concentration 10%. And leached rate of gold in the following cyanidation is over 90%. The parameters of three series fluidized bed reactors exhibit stability during the semi continuous bioleaching of the concentrate. Arsenic in the concentrate can be got rid of 91% after 6 day leaching. Even after 4 days, 82% of arsenic extraction rate was still obtained. The recovery rates of gold are 92% and 87.5% respectively in cyaniding the above bioleached residues. The results will provide a base for further commercial production of gold development.