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用PCR、小型聚丙烯酰胺凝胶电泳和银染法对小卫星区域p33.6(D1S111)位点的扩增片段长度多态性(Amp—FLP)进行分析和对100例无关中国人p33.6位点的等位基因频率进行调查及数据处理,发现该位点核心序列重复数从9到22之间的全部14个等位基因,片段长度分布于435～925bp之间,基因频率为0.5～35.5％,杂合度为76％。对6个家系共22名相关个体进行分析,符合孟德尔遗传定律;对人体各种不同组织DNA进行该位点的分析,显示出高度的一致性。该位点适用于法医学上的个人识别以及亲子鉴定。
The amplified fragment length polymorphism (Amp-FLP) of p33.6 (D1S111) in the small satellite region was analyzed by PCR, mini-polyacrylamide gel electrophoresis and silver staining, and 100 unrelated Chinese p33 were analyzed. 6 locus allele frequencies were investigated and data processing found that the core sequence repeats from 9 to 22 between all 14 alleles, fragment length distribution between 435 ~ 925bp, the gene frequency of 0.5 ~ 35.5%, the heterozygosity is 76%. A total of 22 related individuals from 6 families were analyzed in accordance with Mendelian laws of inheritance; analysis of this site in various human tissue DNA showed a high degree of agreement. The site is suitable for forensic personal identification and paternity testing.