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目的观察β-榄香烯,碘油不同给药方式对兔肝移植瘤残余肿瘤细胞凋亡、增殖的影响。方法34只家兔肝内肿瘤种植后2周经MRI检查证实后,随机分为对照组:A组(6只生理盐水2ml10min);治疗组:B组(7只碘油单纯栓塞0.5ml10min),C组(7只β-榄香烯冲击灌注50mg10min),D组(7只β-榄香烯持续灌注50mg5hour),E组(7只碘油0.5ml+β-榄香烯50mg栓塞灌注10min)5组。实验兔介入治疗后2周处死,取全部肿瘤组织,采用原位末端标记法检测肿瘤细胞的凋亡指数,免疫组化方法测定肿瘤细胞增殖细胞核抗原的表达。结果各组的凋亡指数和增殖指数分别为1.63±0.24,2.35±0.73,1.94±0.28,2.41±0.40,3.50±0.54和77.3±5.9,57.1±6.8,73.4±6.7,63.0±9.6,38.0±5.0。两者存在负相关。结论1.β-榄香烯持续动脉灌注治疗效果好于一次性冲击灌注;2.β-榄香烯碘油栓塞治疗比单纯碘油栓塞及β-榄香烯持续动脉灌注效果好,可以取得较高的凋亡指数和较低的增殖指数。3.促进肿瘤细胞凋亡,抑制肿瘤细胞增殖是肝动脉化疗栓塞术抗肿瘤作用的分子机制之一。
Objective To observe the effects of different administration modes of β-elemene and lipiodol on the apoptosis and proliferation of residual tumor in rabbit liver xenografts. Methods 34 rabbits were intrahepatic liver cancer 2 weeks after implantation confirmed by MRI examination, then randomly divided into control group: group A (6 saline 2ml10min); treatment group: group B (7 lipiodol simple embolization 0.5ml10min) Group C (7-β-elemene shock infusion 50mg10min), Group D (7β-elemene continuous infusion 50mg5hour), Group E (7iodol oil 0.5ml + β-elemene 50mg embolization for 10min) 5 groups. The experimental rabbits were sacrificed 2 weeks after interventional therapy, and all the tumor tissues were taken. The apoptosis index of tumor cells was detected by in situ end-labeling, and the expression of proliferating cell nuclear antigen was detected by immunohistochemistry. Results The apoptosis index and proliferation index in each group were 1.63 ± 0.24, 2.35 ± 0.73, 1.94 ± 0.28, 2.41 ± 0.40, 3.50 ± 0.54 and 77.3 ± 5.9, 57.1 ± 6.8, 73.4 ± 6.7, 63.0 ± 9.6, 38.0 ± 5.0. There is a negative correlation between the two. The effect of β-elemene continuous arterial infusion is better than that of one-time impact perfusion.2.β-elemene lipiodol embolization is more effective than simple iodized oil embolization and β-elemene continuous arterial perfusion, which can be achieved Higher apoptotic index and lower proliferation index. 3. Promote tumor cell apoptosis and inhibit tumor cell proliferation is one of the molecular mechanisms of anti-tumor effect of transcatheter arterial chemoembolization.