论文部分内容阅读
作者用不连续密度梯度离心法与抗体被动选择相结合,用CD16单克隆抗体进行被动选择,以纯化嗜酸性细胞,得到高纯度、高含量的制品.用白细胞分离法取健康献血者白细胞成份,通过3次离心去除血小板.用磷酸盐缓冲液悬浮白细胞,取33ml悬液加16ml淋巴细胞分离液,密度为1.077～1.080,置于50ml锥形离心管中离心,可将单核细胞分离.用Hank’s平衡缓冲液(HBSS)洗粒细胞两次后,每单位体积的压积细胞用2～3倍体积
The authors used discrete density gradient centrifugation combined with passive antibody selection, passive selection with CD16 monoclonal antibody to purify eosinophils to obtain high-purity, high-content products.White blood cells isolated from white blood cells of healthy blood donors, The platelets were removed by centrifugation three times, and the leukocytes were suspended in phosphate buffered saline, and 33 ml of the suspension and 16 ml of lymphocyte fraction were separated at a density of 1.077-1.080 and centrifuged in a 50 ml conical centrifuge tube to separate the mononuclear cells After washing the granulocytes twice with Hank’s Balanced Buffer (HBSS), the cells per unit volume are compressed with 2 to 3 volumes