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连云港市第二人民医院试产供分离淋巴细胞的1%“细胞离净”(Separicin,以下简称S液),与上海试剂二厂生产的9%聚蔗糖(Ficoll,以下简称F液)均和泛影葡胺配制成比重为1.077的应用液作对比试验,比较淋巴细胞分离后的回收率、纯度、丢失率、淋巴细胞分离层中红细胞污染率等,以检测分离效果。并用细胞存活测定、单层原代细胞培养的细胞毒性试验、自然花瓣形成试验及PHA淋巴细胞转化试验等,测定对淋巴细胞的毒性作用,结果见表。
Lianyungang Second People’s Hospital pilot production for the separation of 1% of lymphocytes “cell from the net” (Separicin, hereinafter referred to as S liquid) and Shanghai Reagent Factory production of 9% Ficoll (hereinafter referred to as F liquid) and Diatrizoate meglumine was formulated into a specific gravity of 1.077 for the application of liquid comparison test to compare the recovery of lymphocytes after isolation, purity, loss rate, lymphocyte separation layer of red cell contamination rate, etc., to test the separation effect. The cytotoxicity against lymphocytes was measured by cell viability assay, cytotoxicity test of single-layer primary cell culture, natural petal formation test and PHA lymphocyte transformation test, and the results are shown in the table.