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为阐释干旱地区内陆湖泊微生物群落对营养盐和盐双重胁迫的响应机制,针对干旱、半干旱地区许多湖泊水体受咸化与富营养化双重影响的特点,通过454高通量测序16S rRNA基因和多元统计分析,研究水体营养盐和盐的添加对细菌群落结构、多样性变化的影响.结果表明,盐与营养盐的输入引起了水体中细菌群落组成和多样性的显著变化,具体表现为:1随着盐度(以w计)的升高,α-变形菌纲(Alphaproteobacteria)的相对丰度显著增加,从对照处理的16.0%±4.6%增至SO(贫营养-盐水)处理的39.8%±18.1%;但会受到水体营养水平的制约.2富营养水平下,微咸条件(盐度为1.5‰)有利于细菌类群丰富度的增加,BE-30(微咸-富营养处理培养30 d样品)的Shannon-Wiener多样性指数(4.62)显著高于FE-30(淡水-富营养处理培养30 d样品,2.08)和SE-30(高盐-富营养处理培养30 d样品,1.69);3高盐(盐度为3.0‰)条件下,随着水体中营养盐含量的增加,喜营养的嗜盐细菌类群大量繁殖(SE-30中无法分类的Comamonadaceae科的相对丰度达到69.3%),因而降低了水体中细菌群落的多样性.研究显示,α-变形菌纲和无法分类的Comamonadaceae科对盐与营养盐的变化较为敏感,可作为干旱区湖泊咸化和富营养化过程的参考类群.
In order to elucidate the response mechanism of microbial communities in inland lakes to salt and salt stress in arid areas, aiming at the characteristics of salinity and eutrophication in many lakes in arid and semi-arid areas, the 16S rRNA gene And multivariate statistical analysis were conducted to study the effects of nutrient and salt addition on bacterial community structure and diversity.The results showed that the input of salt and nutrient salts caused significant changes in bacterial community composition and diversity in water body, : 1 As the salinity (in w) increased, the relative abundance of Alphaproteobacteria increased significantly from 16.0% ± 4.6% of control treatment to SO (oligotrophic-saline) -treated 39.8% ± 18.1%, but it was restricted by the nutrient level of water body.2 Under the eutrophication level, the slight saltiness condition (salinity of 1.5 ‰) was beneficial to the increase of the bacterial population richness, but BE-30 The Shannon-Wiener diversity index (4.62) of cultivating 30 d samples was significantly higher than that of FE-30 (freshwater-eutrophication 30 d samples, 2.08) and SE-30 (high salt-eutrophication culture 30 d samples, 1.69); 3 high salt (salinity 3.0 ‰) conditions, with the water With the increase of nutrient content, the population of hi-eutrophic halophilic bacteria multiplied (the relative abundance of unclassified Comamonadaceae in SE-30 reached 69.3%), thus reducing the diversity of bacterial communities in water.The results showed that α- The Proteobacteria and the unclassified Comamonadaceae families are more sensitive to the changes of salt and nutrients and can be used as a reference taxon for salinization and eutrophication of arid lakes.