在碱性介质中，铜氨络离子会催化过氧化氢与土霉素、甲烯土霉素或强力霉素的化学发光反应，表面活性剂溴化十六烷基三甲基铵（ＣＴＭＡＢ）对该反应具有增敏作用．发光强度与土霉素、甲烯土霉素或强力霉素的含量分别在８．０×１０－８～８．０×１０－６ｍｏｌ／Ｌ、５．０×１０－８～５．０×１０－６ｍｏｌ／Ｌ和１．０×１０－７～１．０×１０－５ｍｏｌ／Ｌ的范围内分两个量程呈线性关系．７次平行测定的相对标准偏差分别为１．８％～４．１％、２．４％～３．５％和２．５％～４．０％．标准加入的回收率为９９．６％～１０１．４％、９９．１％～１０２．３％和９９．４％～１０１．４％．应用于尿液中这３种四环素类药物的回收测定，结果满意 In alkaline media, cuprammonium ions catalyze the chemiluminescent reaction of hydrogen peroxide with oxytetracycline, methacycline or doxycycline, the surfactant cetyltrimethylammonium bromide (CTMAB) Sensitization of the reaction. Luminescence intensity and oxytetracycline, methacycline or doxycycline were 8.0 × 10-8 ~ 8.0 × 10-6mol / L, 5.0 × 10-8 ~ 5.0 × 10-6mol / L and 1.0 × 10-7 ~ 1.0 × 10-5mol / L within two ranges of a linear relationship. The relative standard deviations of the seven parallel determinations were 1.8% -4.1%, 2.4% -3.5% and 2.5% -4.0%, respectively. The standard recovery rate was 99.6% ~ 101.4%, 99.1% ~ 102.3% and 99.4% ~ 101.4%. Used in the urine of these three tetracycline drug recovery determination, the results are satisfactory