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目的 研究膀胱癌细胞凋亡与膀胱癌多药耐药的关系。方法 用电镜观察几种临床常用的化疗药物阿霉素、顺铂和丝裂霉素在体外诱导膀胱癌细胞的凋亡的形态学特征 ,免疫组化测定pgp糖蛋白的表达 ,应用MTT法检测阿霉素、顺铂和丝裂霉素的杀伤率。结果 16例患者中 9例有效地诱导出细胞凋亡 ,7例未诱导出凋亡 ,电镜观察显示 ,患者的膀胱癌细胞对化疗药物阿霉素、顺铂和丝裂霉素敏感时 ,其细胞呈特异性的凋亡形态学特征改变 :染色质聚集于核膜呈块状 ,扩张的线粒体 ,嵴移位膨胀 ,凋亡细胞被周围细胞吸收。体外检测显示耐药患者肿瘤细胞抑制率低于 3 0 %。结论 研究结果提示多药耐药的产生与膀胱癌细胞凋亡受到抑制有关。
Objective To study the relationship between bladder cancer cell apoptosis and multidrug resistance in bladder cancer. Methods Electron microscopy was used to observe the morphological characteristics of several commonly used chemotherapeutic drugs, doxorubicin, cisplatin and mitomycin, in vitro to induce the apoptosis of bladder cancer cells. The expression of pgp glycoprotein was detected by immunohistochemistry and the expression of pgp glycoprotein was detected by MTT assay Adriamycin, cisplatin and mitomycin killing rate. Results Of the 16 patients, 9 showed apoptosis effectively and 7 did not induce apoptosis. Electron microscopy showed that when the bladder cancer cells were sensitive to chemotherapeutic drugs doxorubicin, cisplatin and mitomycin, Cell morphological changes were characterized by apoptosis: chromatin gathered in the nuclear membrane was massive, expanding mitochondria, cristae displaced expansion, apoptotic cells were absorbed by the surrounding cells. In vitro tests showed that the tumor cell inhibition rate of drug-resistant patients was less than 30%. Conclusion The results suggest that multidrug resistance is related to the inhibition of apoptosis of bladder cancer cells.