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[Objectives]To optimize extraction process of total flavonoids from Akebia trifoliata( Thunb.) Koidz.,so as to provide references for development and use of Akebia trifoliata( Thunb.) Koidz. [Methods]The extraction rate of total flavonoids of Akebia trifoliata( Thunb.)Koidz. was taken as observation indicator. On the basis of single factor experiment,central composite design( CCD) was used to evaluate the effects of the extraction temperature,extraction time,and ethanol concentration on the extraction process. Multiple linear regression and binomial fitting were used,and response surface methodology( RSM) was used to select the optimum extraction process. [Results] The optimum extraction process conditions for total flavonoids of Akebia trifoliata( Thunb.) Koidz. was extraction temperature: 83. 92 ℃; extraction time:96. 47 min; ethanol concentration: 63. 92%; extraction times: two times; solid to liquid ratio: 1 ∶ 20; extraction rate of total flavonoids:4. 55%. [Conclusions] The central composite design-response surface methodology( CCD-RSM) is simple,convenient,and feasible for extraction of total flavonoids from Akebia trifoliata( Thunb.) Koidz.,and the prediction results are reliable.
[Objectives] To optimize extraction process of total flavonoids from Akebia trifoliata (Thunb.) Koidz., So as to provide references for development and use of Akebia trifoliata (Thunb.) Koidz. [Methods] The extraction rate of total flavonoids of Akebia trifoliata On the basis of single factor experiment, central composite design (CCD) was used to evaluate the effects of the extraction temperature, extraction time, and ethanol concentration on the extraction process. Multiple linear (Thunb.) Koidz. regression and binomial fitting were used, and response surface methodology (RSM) was used to select the optimum extraction process. [Results] The optimum extraction process conditions for total flavonoids of Akebia trifoliata (Thunb.) Koidz. was extraction temperature: 83. 92 Extraction time: 96. 47 min; ethanol concentration: 63. 92%; extraction times: two times; solid to liquid ratio: 1: 20; extraction rate of total flavonoids: 4.55% Central composite design-response surface methodology (CCD-RSM) is simple, convenient, and feasible for extraction of total flavonoids from Akebia trifoliata (Thunb.) Koidz., and the prediction results are reliable.