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目的 了解重组的日本血吸虫中国大陆株脂肪酸结合蛋白 (r Sj- FABPc)的特性及预测其抗原表位。方法 以PCR方法从 Sj- c DNA文库中扩增 Sj- FABPc基因片段 ,并亚克隆于载体 p GEM- T,对其核苷酸序列进行测定。以 GOL DKEY○R软件和 Swiss- Model数据库分析 Sj- FABPc的核苷酸序列和其编码的蛋白质特性 ,预测重组抗原的表位。结果 核酸序列分析证明 ,克隆基因确为 Sj- FABPc,并由此推导出 Sj- FABPc理论氨基酸顺序 ,以该序列的分析表明 ,重组 Sj- FABPc抗原具有 3个抗原表位区和三个可能的脂肪酸结合表位。结论 Sj- FABPc抗原表位可能与脂肪酸结合表位的主要片段为同一肽段。
Objective To understand the characteristics of recombinant Sj-FABPc in Chinese mainland and to predict its epitopes. Methods Sj-FABPc gene fragment was amplified from Sj-c DNA library by PCR and subcloned into vector p GEM-T. The nucleotide sequence of Sj-FABPc gene was determined. The nucleotide sequence of Sj-FABPc and its encoded protein properties were analyzed using the GOL DKEY® R software and the Swiss-Model database to predict the epitope of the recombinant antigen. Results Nucleic acid sequence analysis proved that the cloned gene was indeed Sj-FABPc, and deduced the theoretical amino acid sequence of Sj-FABPc. Analysis of this sequence showed that the recombinant Sj-FABPc antigen has three epitope regions and three possible Fatty acid binding epitopes. Conclusion Sj-FABPc epitope may be the same fragment as the main fragment of fatty acid binding epitope.