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目的:观察HGF在人牙周膜成纤维细胞中的表达及探讨转化生长因子-β1(TGF-β1)、白细胞介素-1β(IL-1β)与HGF之间的关系。方法:分别采用IL-1β梯度浓度、最佳干预浓度和TGF-β1梯度浓度干预第5代人牙周膜成纤维细胞,并运用酶联免疫技术检测人牙周膜成纤维细胞的上清液中HGF的表达。结果:经IL-1β单独作用,人牙周膜成纤维细胞的上清液中HGF的含量较空白对照组高,并优选出IL-1β的最佳干预浓度为10 ng/mL。当一定浓度的TGF-β1和10 ng/mL的IL-1β联合干预人牙周膜成纤维细胞,该细胞上清液中HGF的含量较单独IL-1β作用组低。结论:TGF-β1能够抑制IL-1β诱导的人牙周膜成纤维细胞分泌HGF。
OBJECTIVE: To observe the expression of HGF in human periodontal ligament fibroblasts and to explore the relationship between transforming growth factor-β1 (TGF-β1), interleukin-1β (IL-1β) and HGF. Methods: The 5th generation human periodontal ligament fibroblasts were intervened by gradient concentration of IL-1β, the best intervention concentration and the concentration of TGF-β1, and the supernatant of human periodontal ligament fibroblasts was detected by enzyme-linked immunosorbent assay HGF expression. Results: The level of HGF in the supernatant of human periodontal ligament fibroblasts was higher than that in the blank control group by IL-1β alone, and the optimal concentration of IL-1β was 10 ng / mL. When a certain concentration of TGF-β1 and 10 ng / mL of IL-1β were combined to interfere with human periodontal ligament fibroblasts, the content of HGF in the cell supernatant was lower than that in IL-1β alone group. Conclusion: TGF-β1 can inhibit IL-1β-induced human periodontal ligament fibroblasts to secrete HGF.