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Using a newly generated monocional antibody (2E6) against human B7-H3,we explored the expression of themolecule on dendritic cells derived from monocytes (Mo-DCs).Its expression was examined by means ofimmunostaining and flow cytometric (FCM) analysis.The results showed that B7-H3 was expressed in the courseof Mo-DC maturation induced with interleukin 4 (IL-4) and granulocyte/macrophage colony-stimulating factor(GM-CSF).The expression could be detected at all the stages of Mo-DC differentiation,and remained at a quitestable level.Interestingly,B7-H3 was not expressed by T ceils and B cells,even these ceils were activatedrespectively by PHA or PWM.A weak expression could be detected on resting monocytes.These data showed thatconstitutive expression of B7-H3 at a high level was found on imDCs and mDCs derived from monocytes.Due to noexpression on T cells and B cells,we speculate that B7-H3 might be another valuable molecule marker for Mo-DCs.Cellular & Molecular Immunology.2005;2(4):307-311.
Using a newly generated monocional antibody (2E6) against human B7-H3, we explored the expression of these molecules on dendritic cells derived from monocytes (Mo-DCs) .Its expression was examined by means of immunostaining and flow cytometric (FCM) analysis.The results showed that B7-H3 was expressed in the course of Mo-DC maturation induced with interleukin 4 (IL-4) and granulocyte / macrophage colony-stimulating factor differentiation, and remained at a quite level of accuracy.Interestingly, B7-H3 was not expressed by T ceils and B cells, even these ceils were activatedrespectively by PHA or PWM. A weak expression could be detected on resting monocytes.These data showed that consitutive expression of B7-H3 at a high level was found on imDCs and mDCs derived from monocytes. Due to noexpression on T cells and B cells, we speculate that B7-H3 might be else valuable molecule marker for Mo-DCs. Cellular & Molecular Immunology. 2005 ; 2 (4): 307- 311.