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背景:一氧化氮合酶(nitricoxidesynthase,NOS)广泛分布于中枢神经系统并调节神经活动,但鲜见在中枢神经脊上水平有关NOS参与内脏神经传入信息传递的报道。目的:探查三叉神经脊束间质核(interstitialnucleusoftrigeminaltract,INV)内内脏传入终末与向臂旁核(parabrachialnucleus,PBN)投射的NOS阳性神经元之间的联系。设计:以实验动物为研究对象,验证性对比观察。单位:一所军医大学解剖学教研室。材料:选择成年SD雄性大鼠10只进行动物试验。干预:分别给PBN及舌咽和迷走神经内注射四甲基罗丹明(tetramethylrhodamine-dextran,TMR),生物素化葡聚糖胺(biotinylateddextran-amine,BDA)并进行NOS免疫组织化学反应,结合激光扫描共聚焦显微镜观察。主要观察指标:观察逆行TMR标记细胞、NOS阳性细胞以及跨神经节BDA标记的传入终末在INV内的重叠分布和三者的相互关系。结果:逆行标记细胞主要位于注射侧的INV,大多属20μm以下的中、小型细胞。NOS阳性细胞与TMR逆行标记细胞分布区域重叠。计数显示:NOS/TMR双标记细胞分别占NOS阳性细胞总数的55%(17/31)和TMR逆行标记细胞总数的34%(17/49)。跨神经节注射BDA标记的内脏神经初级传入终末点状膨体贴近双标记细胞胞体,呈紧密接触状。结论:存在经INV向PBN投射的内脏信息传导
BACKGROUND: Nitric oxide synthase (NOS) is widely distributed in the central nervous system and regulates neural activity, but rare reports of NOS involvement in visceral nerve afferent transmission at the central nervous ridges. Objective: To explore the relationship between visceral afferent terminals in interstitial nucleus of triminaltract (INV) and NOS positive neurons projecting to parabrachial nucleus (PBN). Design: The experimental animals as the research object, confirmatory contrast observation. Unit: An Military Medical University Department of Anatomy. MATERIALS: Ten adult male SD rats were selected for animal testing. Intervention: Intravenous and vaginal injection of tetramethylrhodamine-dextran (TMR) and biotinylateddextran-amine (BDA) into PBN and glossopharyngeal and vagus nerve respectively were performed and NOS immunohistochemical reaction was performed. Combined with laser scanning Confocal microscopy. MAIN OUTCOME MEASURES: The overlap distribution of retrograde TMR-labeled cells, NOS positive cells and trans-ganglion BDA-labeled end-effector in INV and the correlation between them were observed. Results: The retrogradely labeled cells mainly located in the INV at the injection side, most of which were medium and small cells below 20μm. NOS positive cells overlap with TMR retrograde marker cell distribution. Counting showed that NOS / TMR double labeled cells accounted for 55% (17/31) of the total number of NOS positive cells and 34% (17/49) of the total number of TMR retrogradely labeled cells, respectively. Transplantation of BDA-labeled visceral nerve primary afferent end-point parenchyma into BDA-labeled neurons close to the double labeled cell body, in close contact. CONCLUSIONS: There is visceral message transmission via the INV to PBN