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为探讨人脐带血细胞分化为神经样细胞的可行性 ,无菌条件下采集正常人脐血 5 0～ 10 0mL置于加有抗凝剂的采血袋中 ,分离脐血单个核细胞进行培养 ,并加入细胞因子加以诱导分化 ,利用免疫细胞化学方法进行鉴定。结果显示 :脐血单个核细胞能在体外培养中增生分化和表达干细胞特异性抗原神经巢蛋白 (nestin) ,并最终分化为神经元样和神经胶质样细胞。免疫组化染色可见神经元特异性烯醇化酶 (NSE)和胶质原性纤维酸性蛋白 (GFAP)抗原表达。碱性成纤维细胞生长因子 (bFGF)和表皮生长因子 (EGF)协同作用对脐血细胞的分化有促进作用。提示 :脐血单个核细胞具有增生和分化潜能 ,可分化为神经元样及神经胶质细胞样细胞 ,可以作为神经细胞的种子细胞。
To explore the feasibility of human umbilical cord blood cells differentiated into neuron-like cells, normal human umbilical cord blood 50 ~ 10 0mL was collected under aseptic conditions in a blood collection bag with anticoagulant, and cord blood mononuclear cells were isolated and cultured Addition of cytokines to induce differentiation, the use of immunocytochemistry to identify. The results showed that umbilical cord blood mononuclear cells could proliferate and differentiate and express stemin-specific nestin in vitro and eventually differentiate into neuron-like and glial-like cells. Immunohistochemical staining showed neuron-specific enolase (NSE) and glial fibrillary acidic protein (GFAP) antigen expression. Synergistic effect of basic fibroblast growth factor (bFGF) and epidermal growth factor (EGF) can promote the differentiation of umbilical cord blood cells. Tip: Cord blood mononuclear cells have proliferation and differentiation potential, can differentiate into neuron-like and glial-like cells, can be used as the nerve cell seed cells.