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[目的]探讨脂肪酸辅酶A连接酶4(fatty acid CoA ligase 4,FACL4)抑制剂TriacsinC对HepG2细胞增殖和凋亡的影响。[方法]应用免疫细胞化学SP染色法检测FACL4在HepG2细胞中的表达情况;应用不同浓度的FACL4抑制剂TriacsinC处理HepG2细胞,利用MTT比色法、生长曲线测定观察TriacsinC对HepG2细胞增殖的影响;利用HE染色、流式细胞仪观察细胞凋亡。[结果]FACL4阳性表达主要位于胞浆。TriacsinC对HepG2细胞具有明显的生长抑制作用,不同浓度的TriacsinC(500、1000、1500、2000μg/L)处理HepG2细胞72h的抑制率分别为49.99%、54.21%、61.39%和68.07%。TriacsinC能诱导HepG2细胞凋亡。不同浓度(500、1000、1500、2000μg/L)处理HepG2细胞48h后,其凋亡率分别为19.47%、20.4%、20.9%、24.67%,明显高于对照组的1.93%。[结论]FACL4在HepG2细胞中呈高表达;Triac-sinC以时间、剂量依赖方式抑制细胞增殖;TriacsinC以剂量依赖方式诱导细胞凋亡。
[Objective] To investigate the effect of fatty acid CoA ligase 4 (FACL4) inhibitor TriacsinC on the proliferation and apoptosis of HepG2 cells. [Methods] The expression of FACL4 in HepG2 cells was detected by immunocytochemical SP staining. HepG2 cells were treated with different concentrations of FACL4 inhibitor TriacsinC. The effect of TriacsinC on the proliferation of HepG2 cells was observed by MTT assay and growth curve. HE staining and flow cytometry were used to observe apoptosis. [Results] The positive expression of FACL4 mainly located in the cytoplasm. TriacsinC had obvious growth inhibitory effect on HepG2 cells. The inhibitory rates of TriacsinC (500,1000,1500,2000μg / L) treated HepG2 cells for 72h were 49.99%, 54.21%, 61.39% and 68.07%, respectively. TriacsinC can induce HepG2 cell apoptosis. The apoptosis rates of HepG2 cells treated with different concentrations (500,1000,1500,2000 μg / L) for 48 h were 19.47%, 20.4%, 20.9% and 24.67%, respectively, which were significantly higher than those of the control group (1.93%). [Conclusion] FACL4 is highly expressed in HepG2 cells. Triac-sinC inhibits cell proliferation in a time-and dose-dependent manner. TriacsinC induces apoptosis in a dose-dependent manner.