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目的:在国内率先分离、培养肝硬化大鼠贮脂细胞,并用以研究中药抗肝纤维化的作用机理。方法:以~3H-胸腺嘧啶(~3H-TdR)、~H-脯氨酸掺入量及掺入率测定方法,观察肝纤康对正常大鼠离体贮脂细胞和肝硬化大鼠离体贮脂细胞功能的影响。结果:该方能抑制贮脂细胞DNA及胶原合成,抑制程度随药物浓度的升高而增高。结论:肝纤康防治肝硬化的机制之一,可能在于该方抑制贮脂细胞增殖及胶原合成。
OBJECTIVE: To isolate and culture lipid-storage cells from liver cirrhosis rats in China, and to study the mechanism of anti-hepatofibrosis effects of traditional Chinese medicine. Methods: Observe the effect of liver fibrosis on the isolated rat fat-storing cells and liver cirrhosis rats by measuring the ~3H-thymidine (~3H-TdR), ~H-proline incorporation and incorporation rate. Effects of body fat storage cell function. Results: This method can inhibit the synthesis of DNA and collagen in lipid-storage cells. The degree of inhibition increases with the increase of drug concentration. Conclusion: One of the mechanisms of liver fibrosis prevention and treatment of liver cirrhosis may lie in its inhibition of lipid-storing cell proliferation and collagen synthesis.