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在H2SO4介质中,KIO3可氧化吖啶红使之褪色并伴随着吖啶红荧光强度的减弱,但其反应速率较慢。当加入痕量钴(Ⅱ)时,氧化反应明显加快,体系荧光猝灭程度加强,钴(Ⅱ)对该氧化反应具有显著的催化作用。据此建立了一种灵敏度高、选择性好的测定痕量钴(Ⅱ)的催化荧光分析新方法,并研究了该方法的动力学条件。反应体系的激发波长和发射波长分别为530和550nm,催化体系可允许较大量的多种常见离子同时存在。测定钴(Ⅱ)的线性范围为0.3～4.8μg/L,检出限为2.5×10-11g/mL。方法可用于茶叶、人发和面粉等实际样品中痕量钴(Ⅱ)的测定。
In H 2 SO 4 medium, KIO 3 can oxidize acridine red to make it fade with acridine red fluorescence intensity decreased, but the reaction rate is slower. When a trace amount of cobalt (Ⅱ) was added, the oxidation reaction was accelerated obviously, and the fluorescence quenching of the system was strengthened. Cobalt (Ⅱ) had a significant catalytic effect on the oxidation reaction. Based on this, a new method for the determination of trace cobalt (Ⅱ) by catalytic fluorescence spectrometry has been established with high sensitivity and selectivity. The kinetic conditions of this method were also studied. The excitation and emission wavelengths of the reaction system are 530 and 550 nm, respectively. The catalytic system can allow a large number of common ions to coexist. The linear range of cobalt (Ⅱ) was 0.3 ~ 4.8μg / L, and the detection limit was 2.5 × 10-11g / mL. The method can be applied to the determination of trace cobalt (Ⅱ) in real samples such as tea, human hair and flour.