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本文介绍用一个简便的两步法从哺乳动物血清或腹水中提取免疫球蛋白G(IgG)。即首先用辛酸沉淀白蛋白和其他非IgG类蛋白,然后再用硫酸铵沉淀IgG组分。作者认为,此方法的关键在于若辛酸沉淀完全,下一步硫酸铵沉淀IgG时杂质才会减少。为此,须注意以下一些影响因素:(1)血清浓度,血清稀释3～4倍时,白蛋白和非IgG蛋白沉淀完全,沉淀中几乎不含IgG。(2)pH,pH4.5时所有非IgG蛋白可完全沉淀,上清中留有非常纯净的IgG。(3)辛酸浓
This article describes the extraction of immunoglobulin G (IgG) from mammalian serum or ascites using a simple, two-step procedure. That is, albumin and other non-IgG proteins are first precipitated with caprylic acid, and then the IgG fraction is precipitated with ammonium sulfate. The authors believe that the key to this method is that if the caprylic acid is completely precipitated, the impurities will be reduced in the next ammonium sulfate precipitation of IgG. To this end, we must pay attention to the following factors: (1) Serum concentrations, serum dilution of 3 to 4 times, albumin and non-IgG protein precipitation complete, almost no precipitation in the IgG. (2) Complete precipitation of all non-IgG proteins at pH, pH 4.5 with very pure IgG in the supernatant. (3) Caprylic acid concentration