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目的:研究丹参素钠对大鼠脑缺血再灌注(I/R)损伤的保护作用。方法:成年Wistar大鼠50只随机分为模型对照组、低剂量组、中剂量组、高剂量组、阳性对照组,MCAO法建立大鼠脑缺血模型,脑缺血1h,再灌注24h后进行神经缺损症状行为评分;TTC染色法观察脑梗死面积;比色法测定组织总超氧化物歧化酶(t-SOD)活力、谷胱甘肽过氧化物酶(GSH-PX)活力、黄嘌呤氧化酶(XOD)活力、丙二醛(MDA)含量。结果:与模型对照组相比,低剂量组、中剂量组、高剂量组和阳性对照组缺血1h再灌注24h后行为评分降低(P<0.01);脑梗死范围显著减小;t-SOD活力升高(P<0.01);GSH-PX活力升高(P<0.01);XOD活力降低(P<0.01);MDA含量降低(P<0.01)。结论:丹参素钠可以降低脑I/R后对神经的损伤,减小脑梗死范围,提高脑组织抗氧化能力,减少内皮功能降低,从而降低脑I/R对脑组织的损伤。
Objective: To study the protective effect of Danshensu on cerebral ischemia-reperfusion (I / R) injury in rats. Methods: Fifty adult Wistar rats were randomly divided into model control group, low dose group, middle dose group, high dose group, positive control group, MCAO method to establish cerebral ischemia model, cerebral ischemia 1h, 24h after reperfusion The neurological deficit symptoms were scored. The area of cerebral infarction was observed by TTC staining. The activities of t-SOD, glutathione peroxidase (GSH-PX), xanthine Oxidase (XOD) activity, malondialdehyde (MDA) content. Results: Compared with the model control group, the behavioral scores decreased after 1h of reperfusion in low dose group, middle dose group, high dose group and positive control group (P <0.01); the range of cerebral infarction was significantly reduced; t-SOD (P <0.01); the activity of GSH-PX increased (P <0.01); the activity of XOD decreased (P <0.01); the content of MDA decreased (P <0.01). Conclusion: Danshensu sodium can reduce the damage of nerve after I / R of brain, reduce the range of cerebral infarction, improve the antioxidation ability of brain tissue, decrease the function of endothelial cells and reduce the damage of brain I / R.