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背景:有报道参麦注射液具有治疗冠心病与抗肿瘤作用,但其作用机制如何?目的:从对血管生成影响角度探讨参麦抗肿瘤的作用机制。设计:设立对照的实验研究。地点和材料:浙江中医学院分子医学研究所。人癌细胞株购自中国科学院细胞生物学研究所。参麦注射液(10mL/2g):正大青春宝公司。干预:采用MTT法检测参麦注射液对牛血清和肿瘤细胞(SMMC-7721)条件培养液促进的牛主动脉内皮细胞增殖的影响;采用琼脂糖刮除法检测参麦对牛血清和肿瘤细胞(SMMC-7721)条件培养液促进的牛内皮细胞迁移的影响。培养细胞分为对照组、条件培养液组、不同浓度参麦注射液组。主要观察指标:参麦注射液对牛内皮细胞增殖和迁移的影响,参麦对条件培养液促牛内皮细胞迁移的影响。结果:无论是在含100mL/L新生小牛血清培养液中还是在肿瘤细胞条件培养液中,参麦均能明显抑制牛主动脉内皮细胞增殖,且随着剂量增加,抑制作用增强。当参麦的浓度为40mL/L时即可显著抑制牛血清促进的内皮细胞增殖,与对照组相比,差异有显著性意义(t=4.83,P<0.01),20mL/L的参麦即可明显地抑制由条件培养液促进的内皮细胞增殖,与对照组相比,差异有显著性意义(t=5.32,P<0.01)。经20mL/L参麦处理48h后,对内皮细胞迁移的抑制率为58%,与对照组相比差异有显著性意义(t=8.3
Background: It has been reported that Shenmai injection has a therapeutic effect on coronary heart disease and anti-tumor, but its mechanism of action? Purpose: To explore the mechanism of anti-tumor effect of Shenmai from the perspective of the effect of angiogenesis. Design: Establish a controlled experimental study. Location and Materials: Institute of Molecular Medicine, Zhejiang University of Traditional Chinese Medicine. Human cancer cell lines were purchased from the Institute of Cell Biology, Chinese Academy of Sciences. Shenmai Injection (10mL/2g): Zhengda Qingbao Company. Intervention: MTT assay was used to detect the effect of Shenmai injection on the proliferation of bovine aortic endothelial cells stimulated by bovine serum and tumor cell (SMMC-7721) conditioned medium; Shenmai was used to detect bovine serum and tumor cells by agarose scraping method. SMMC-7721) conditioned media promotes the effects of bovine endothelial cell migration. The cultured cells were divided into a control group, a conditioned medium group, and a different concentration of Shenmai injection group. MAIN OUTCOME MEASURES: The effect of Shenmai injection on the proliferation and migration of bovine endothelial cells, and the effect of Shenmai on the migration of bovine endothelial cells induced by conditioned medium. RESULTS: Both in the medium containing 100 mL/L newborn calf serum or in the conditioned medium of the tumor cells, Shenmai could significantly inhibit the proliferation of bovine aortic endothelial cells, and the inhibitory effect was enhanced with increasing dose. When the concentration of Shenmai was 40 mL/L, the proliferation of endothelial cells promoted by bovine serum was significantly inhibited. Compared with the control group, the difference was significant (t=4.83, P<0.01), and 20 mL/L of Shenmai The proliferation of endothelial cells promoted by conditioned medium was significantly inhibited compared with the control group (t=5.32, P<0.01). After treatment with 20 mL/L Shenmai for 48 h, the inhibition of endothelial cell migration was 58%, which was significantly different from the control group (t=8.3).