论文部分内容阅读
目的 研究新型农药吡虫啉对原代培养的大鼠海马神经元细胞培养液内NO(一氧化氮 )含量和细胞内NOS(一氧化氮合酶 )活性的影响。方法 原代培养的大鼠海马神经元成熟后 ,分组加入不同浓度的吡虫啉进行染毒 ,于染毒后 12h、2 4h、4 8h分别在相差显微镜下观察其形态学改变 ,并测定细胞死亡率 ,细胞培养液内NO含量及细胞内NOS的活性。结果 与DMSO对照组相比 ,除 0 ,10 -6mol/L吡虫啉组外 ,随染毒浓度增加 (10 -5～ 10 -3 mol/L)和染毒时间延长 ,出现细胞肿胀、胞体内颗粒增多和突起断截等改变 ,细胞间网络也变稀疏 ;细胞死亡率和细胞培养液NO含量在作用 2 4h后增加 (P <0 0 5 ) ,作用 4 8h继续增加 (P <0 0 1)。细胞内NOS活性在作用 12h于 10 -3 mol/L组首先出现升高 (P <0 0 1) ;作用 2 4h ,10 -5～ 10 -4mol/L组也升高 (P <0 0 1) ,10 -3 mol/L继续升高 (P <0 0 1) ;作用 4 8h ,各浓度组吡虫啉的细胞内NOS活性与 2 4h时相比无明显变化。结论 10 -5～ 10 -3 mol/L浓度的吡虫啉可引起原代培养的大鼠海马神经元细胞培养液内NO含量和细胞内NOS活性的升高 ,这可能与其神经毒性作用有关。
Objective To investigate the effect of a new pesticide, imidacloprid, on NO (nitric oxide) content and intracellular NOS (nitric oxide synthase) activity in cultured primary cultured rat hippocampal neurons. Methods After primary cultured rat hippocampal neurons matured, different concentrations of imidacloprid were added into groups for exposure. Morphological changes were observed under phase contrast microscope at 12h, 24h and 48h after exposure, and the cell death rate , NO content in cell culture medium and intracellular NOS activity. Results Compared with the DMSO control group, with the increase of the concentration (10 -5 ~ 10 -3 mol / L) and the prolongation of exposure time, the cell swelling, The number of cells and the content of NO in cell culture medium increased after 24 hours (P <0.05), and continued to increase 48 hours after treatment (P <0.01) . The intracellular NOS activity increased firstly in 10-3 mol / L group (P <0.01) at 12h, and also increased at 10-4 mol / L group (P <0.01) ) And 10 -3 mol / L (P <0.01). After 48 h of treatment, the intracellular NOS activity of imidacloprid in each concentration group had no significant change compared with that at 24 h. Conclusion Imidacloprid at a concentration of 10 -5 ~ 10 -3 mol / L may induce NO content and intracellular NOS activity in cultured primary cultured hippocampal neurons, which may be related to its neurotoxicity.