对上海地区球根鸢尾进行了病毒病害调查。经检测初步确定球根鸢尾普遍存在鸢尾重花叶病毒、鸢尾轻花叶病毒、水仙潜隐病毒３种病毒的复合侵染。对带毒种球进行茎尖组培脱毒，得出了茎尖诱导成芽、芽成球及小球生根的培养基配方。将组培苗在试管苗、成球苗、移栽苗等不同时期进行病毒检测，结果表明，小于１．０ｍｍ的茎尖诱导的幼苗可脱去上述３种病毒。 Virus disease investigation was conducted on the bulbous Iris in Shanghai. The test initially identified Iris bulbar common Iris heavy mosaic virus, Iris light mosaic virus, Narcissus latent virus three kinds of virus infection. The shoot tip tissue culture virus-free bulbs were obtained, and the medium recipe of bud induction, shoot bud formation and pellet rooting was obtained. The tissue culture seedlings were tested in different stages such as test-tube seedlings, seedling seedlings and transplanting seedlings. The results showed that the seedlings induced by shoot tips less than 1.0mm could take off the above three viruses.