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本研究报道了裸露心肌标本的制备及研究肌钙蛋白Ca~(2+)敏感性的技术方法。10mmol/L EGTA裸露液浸浴大鼠右室乳头肌,其肌膜通透性明显增高,表现为去除舒张液中ATP和磷酸肌酸后,心肌立即挛缩。最适裸露时间为24h。此时最大Ca~(2+)激活张力(T_(max)最高;收缩和舒张至50％T_(max)所需时间T_(1/2)和R_(1/2)最短。张力-pCa(Ca~(2+)摩尔浓度的负对数)关系曲线是S形,且同一标本先后两次实验的张力-pCa关系及50％T_(max)时的pCa(pCa_(50))均无明显差别,而茶碱可使张力-pCa关系左移,pCa_(50)增高。表明高通透心肌标本功能稳定,且可反映肌钙蛋白Ca~(2+)敏感性的改变。
This study reports the preparation of naked myocardial specimens and the method of studying the sensitivity of troponin Ca ~ (2+). Right ventricular papillary muscle of 10mmol / L EGTA exposed bath immersion bath, the permeability of the muscle membrane was significantly increased, the performance of the diastolic fluid removal of ATP and creatine phosphate, the immediate immediate contracture. The best exposure time is 24h. The maximal Ca 2+ activation tension (T max) was the highest at this time, and the shortest time T 1/2 (1/2) and R 1/2 was the shortest between contraction and relaxation to 50% T max Tension-pCa The negative logarithm of Ca 2+ concentration was S-shaped, and there was no obvious relationship between the tension-pCa and the pCa (50) at 50% T max of the same experiment While theophylline shifted the tension-pCa relationship to the left and increased the pCa_ (50), indicating that the hyperpermeable myocardium is stable and can reflect the change of Ca 2+ sensitivity.