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研究目的:檀香(Santalum album Linn.)为檀香科(Santalaceae)檀香属(Santalum)树种,属于常绿半寄生小乔木,其心材为传统名贵中药。檀香与其寄主植物假蒿通过根端的吸盘相连,探讨檀香与其宿主之间内生真菌的关系具有重要意义。创新要点:假蒿(Kuhnia rosmarinifolia Vent.)是幼龄檀香很好的寄主。目前已经对许多药用植物的内生真菌的分离和鉴定进行了大量研究,但是关于檀香和假蒿之间关系的研究报道较少。本研究首次探讨了檀香及其宿主植物假蒿根中内生真菌的数量和种类,为进一步研究两者之间的关系提供一些有用的信息。研究方法:采用组织块分离法从檀香的根和其宿主植物假蒿的根部材料分离内生真菌,并采用形态学鉴定和分子鉴定手段对分离的内生真菌进行鉴定。重要结论:从檀香的根和其宿主植物假蒿的根中160个组织块中分离和鉴定了共25个真菌类群,其中青霉属真菌Penicillium sp.1为檀香根中优势菌,镰孢属真菌Fusarium sp.1为假蒿根中优势菌。特别有意思的是两种植物根中没有分离到明显相同的内生真菌。
Purpose: Sandalwood (Santalum album Linn.) Is a species of Santalum of Santalaceae, belonging to the evergreen semi-parasitic small tree whose heartwood is a traditional precious Chinese traditional medicine. Sandalwood and its host plant Artemisia through the root of the sucker connected to explore the relationship between sandalwood and its host endophytic fungi is of great significance. Innovation points: Artemisia (Kuhnia rosmarinifolia Vent.) Is a good host of young sandalwood. Numerous studies have been conducted on the isolation and identification of endophytic fungi in many medicinal plants, but few reports have been reported on the relationship between sandalwood and Artemisia. This study, for the first time, explores the numbers and types of endophytic fungi in the roots of sandalwood and its host plant Artemisia selengensis, providing some useful information for further studying the relationship between the two. Methods: The endophytic fungi were isolated from the root of sandalwood and the root material of the host plant Artemisia annua using tissue block separation method. The isolated endophytic fungi were identified by morphological and molecular identification methods. Important conclusions: A total of 25 fungal groups were isolated and identified from 160 tissue blocks of the root of sandalwood and its host plant Artemisia. Penicillium sp.1 was the dominant species of sandalwood root, Fusarium sp.1 fungi is the dominant bacteria in the root of Artemisia. Of particular interest is the absence of distinctly identical endophytic fungi isolated from both plant roots.