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Objective: To study the effect of three different methods such as medicine, acupuncture and laser acupuncture on the levels of interleukin 2 (IL-2), inter-leukin 6 (IL-6) and transforming growth factor-β1 (TGF-β1) in rats serum with chronic atrophic gastritis. Methods: 9 rats were randomly selected from 60 rats as normal con-trol group, and the other rats were used to replicate the animal model of chronic atrophic gastritis with comprehensive method, 5 rats were randomly inspected at the 8th week in the process of creating model for gastric mucosa pathological examination;when gastric mucosa shows CAG symptoms such as various degree of congestion, bleeding, atrophy, metaplasia, the model rats were randomly divided into model group, medicine group, acupuncture group and laser acupuncture group. The rats of medicine group were chron-ically administered with yanshenjianwei capsule and Kangfuxin Liquid; the groups of acupuncture and laser acupuncture selected Zusanli acupuncture as acupuncture points,after the treatment for 14 d, blood was removed, double antibody sandwich en-zyme linked immunosorbent assay (ELISA) was used to detect the levels of IL-2 , IL-6 and TGF-β1 in serum. Results: Compared with normal group, the contents of IL-2, IL-6 and TGF-β1 in serum of model rats increased (P