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目的探究miR-125a-3p与p53之间的关系及miR-125a-3p在老年性白内障中的调控作用。方法通过对取自白内障患者的晶状体进行microRNA microarray来发现表达有差异的microRNA,再通过靶基因预测,找到调控p53的microRNA;通过目标microRNA的类似物体外瞬转来研究对p53的调控作用。结果经过microRNA microarray扫描后,得到近169个有差异的microRNA,经过靶基因预测筛选出miR-125a-3p;通过miR-125a-3p类似物的体外转染可抑制p53的表达,进而抑制晶状体上皮细胞凋亡。结论 miR-125a-3p可能与白内障的发病机制有关。
Objective To investigate the relationship between miR-125a-3p and p53 and the regulatory role of miR-125a-3p in senile cataract. Methods MicroRNA microarray was used to detect differentially expressed microRNAs in lens from cataract patients. The target microRNAs were used to find the microRNAs that regulate p53. The regulation of p53 was also studied by transient in vitro analogs of target microRNAs. Results After microRNA microarray scanning, nearly 169 differential microRNAs were obtained and miR-125a-3p was screened out by target gene prediction. The in vitro transfection of miR-125a-3p analogs could inhibit the expression of p53 and then inhibit the growth of lens epithelium Apoptosis. Conclusion miR-125a-3p may be related to the pathogenesis of cataract.