用一株从希腊病犬骨髓分离的婴儿利什曼原虫的前鞭毛体免疫正常BALB/c小鼠,每15天皮下注射10~6个原虫,共3次,一月后动物出现最高抗体滴度,融合前4天再以2×10~7个原虫腹腔注射加强免疫。融合按Galfre等(1977)的方法进行,融合细胞用HA培养基进行选育,用IIF筛选杂交瘤,阳性杂交瘤经有限稀释法克隆后用IIF再筛选。选 Normal BALB / c mice were immunized with a promastigotes of Leishmania infantum isolated from the bone marrow of a Greek sick dog and subcutaneously injected 10 to 6 protozoa every 15 days for three times. The highest antibody titer appeared after one month , 4 days before fusion with 2 × 10 ~ 7 protozoal intraperitoneal injection to enhance immunity. Fusion was carried out as described by Galfre et al. (1977). The fused cells were selected with HA medium and screened for hybridoma with IIF. The positive hybridomas were cloned by limiting dilution and then screened with IIF. selected