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本工作采用大鼠垂体前叶（ＡＰ）细胞原代培养方法，以３ＨＴｄＲ掺入率反映细胞增殖水平，研究了ＩＬ１和ＩＬ６对ＡＰ细胞增殖的影响。结果表明：（１）ＩＬ１（１－１００ｎｇ／ｍｌ）促进雄性大鼠和雌性大鼠ＡＰ细胞的增殖。（２）低浓度的ＩＬ６（０．１ｎｇ／ｍｌ）抑制雄性大鼠的ＡＰ细胞的增殖，而较高浓度的ＩＬ６（１－１０ｎｇ／ｍｌ）则表现为刺激作用。（３）ＩＬ６（０．１－１０ｎｇ／ｍｌ）促进雌性大鼠ＡＰ细胞的增殖。上述结果说明ＩＬ１和ＩＬ６除直接调控ＡＰ细胞的分泌外，也参与调节ＡＰ细胞增殖活动。
This work using rat primary anterior pituitary (AP) cell primary culture method to 3H TdR incorporation rate reflects the level of cell proliferation, IL 1 and IL 6 on AP cell proliferation. The results showed that: (1) IL 1 (1-100ng / ml) to promote male rat and female rat AP cell proliferation. (2) Low concentrations of IL 6 (0.1ng / ml) inhibited the proliferation of AP cells in male rats, and higher concentrations of IL 6 (1-10ng / ml) showed a stimulating effect. (3) IL 6 (0.1-10ng / ml) to promote the proliferation of female rat AP cells. The above results show that IL 1 and IL 6 in addition to directly regulate the secretion of AP cells, but also involved in regulating AP cell proliferation.