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目的探寻导致蛋白印迹法(western blotting,WB)检测结果呈“HIV抗体不确定”可能的生物学因素。方法检测经WB确证后结果为“HIV抗体不确定”病例乙型肝炎(type B hepatitis,HBV)、丙型肝炎(type Chepatitis,HCV)、梅毒螺旋体(treponemiasis,TP)、HTLV-I/II(human T cell leukemia virus I/II,HTLV-I/II)的感染状况及癌胚抗原(carcino-embryonic antigen,CEA)、抗核抗体(antinuclear antibody,ANA)、甲胎蛋白(α-fetoprotein,AFP)、β2微球蛋白(β2-microglobulin,β2-MG)在血液中的含量,并与文献资料报道的普通人群/健康人比较。结果“HIV抗体不确定”病例HCV抗体初筛阳性率、TP抗体阳性率和HTLV-I/II抗体的初筛阳性率均高于普通人群;22.22%的“HIV抗体不确定”病例血液中ANA含量高于普通人群,呈阳性或可疑;9.27%的“HIV抗体不确定”非孕产妇病例血液中AFP含量高于普通人群。结论“HIV抗体不确定”的产生可能与病例感染HCV、TP、HTLV-I/II,病例体内ANA、AFP水平升高有关。
Objective To explore the possible biological factors that cause the result of western blotting (WB) test to be “indeterminate HIV antibody”. Methods The results of WB confirmation were “hepatitis B virus (HBV), type Chepatitis (HCV), treponemiasis (TP), HTLV-I / (CEA), antinuclear antibody (ANA), α-fetoprotein (IL-2) in human T cell leukemia virus I / II (HTLV- , AFP, β2-microglobulin (β2-MG) in the blood, and compared with the general population / healthy people reported in the literature. Results The positive rate of HCV antibody, the positive rate of TP antibody and the positive rate of HTLV-I / II antibody were higher in the cases of ”HIV antibody uncertain“ than in the general population; 22.22% ”HIV antibody uncertain“ ANA levels in the blood of the cases were higher than the general population, positive or suspicious; 9.27% of the ”non-HIV antibody uncertain“ blood levels of AFP in non-pregnant women than in the general population. Conclusion The occurrence of ”HIV antibody uncertainty" may be related to the cases of HCV, TP, HTLV-I / II, and the increase of ANA and AFP in cases.