目的 :观察人内皮抑素对小鼠肺腺癌LA795生长和转移的抑制作用。方法 :对重组人内皮抑素高效表达克隆 pCX的表达产物进行纯化 ,得到重组人内皮抑素 (rhES)。用亲和层析及胰弹性蛋白酶消化法从过期人血浆纯化得到人血管抑素(hAS)。将接种LA795肺腺癌细胞的T739小鼠随机分成 3组 ,分别给予rhES ,hAS或等体积PBS皮下注射 ,1次 /日 ,共 14d。观察 3组肿瘤生长情况、肺湿重、肺表面转移结节数、动物生存期 ,分别进行 q检验。 结果 :rhES组及hAS组肿瘤生长缓慢 ,8d后肿瘤逐渐回缩 ;肺湿重、肺表面转移结节数明显减少 ,动物生存期明显延长。结论 :rhES与hAS均可明显抑制LA795所致的小鼠实验性肿瘤的生长与转移 ,延长动物的生存期。 Objective: To observe the inhibitory effect of human endostatin on the growth and metastasis of lung adenocarcinoma LA795 in mice. Methods: The expression product of recombinant human endostatin highly expressing clone pCX was purified to obtain recombinant human endostatin (rhES). Human angiostatin (hAS) was purified from the expired human plasma by affinity chromatography and pancreatic elastase digestion. T739 mice inoculated with LA795 lung adenocarcinoma cells were randomly divided into 3 groups, which were given rhES, hAS or equal volume of PBS subcutaneously once a day for 14 days. Three groups of tumor growth, lung wet weight, number of lung surface metastasis nodules, animal survival were observed, respectively q test. Results: The tumor growth of rhES group and hAS group was slow. After 8 days, the tumor gradually contracted. The wet weight of lung and the number of pulmonary nodules on the surface of lung decreased obviously, and the survival time of the animal was obviously prolonged. Conclusion: Both rhES and hAS can significantly inhibit the growth and metastasis of experimental mice induced by LA795 and prolong the survival of animals.