油菜花药培养研究始于七十年代,1975年我国从白菜型油菜(Brassica。campestris)中成功地诱导出了花粉植株,随后又获得了甘蓝型油菜(B.napus)的花粉植株。所采用的方法通过诱导愈伤组织的途径来获得花粉植株的。但由于使用的培养基中激素浓度较高而蔗糖浓度又偏低,导致花丝、药隔、药壁的体细胞极易产生愈伤组织,不仅给油菜花粉植株的诱导与鉴定带来了很大的干扰,而且造成花粉植株的诱导率远较水稻、烟草、小麦等 Rape anther culture research began in the seventies. In 1975, pollen plants were successfully induced from Brassica campestris in China and pollen plants of B. napus were obtained subsequently. The method used to obtain the pollen plant is by way of callus induction. However, due to the high concentration of hormone and the low sucrose concentration in the culture medium, the somatic cells of the filament, the septum and the paraffin wall are easy to produce callus, which not only brings about great induction and identification of the rape pollen plants Of the interference, but also caused the induction rate of pollen plants far more than rice, tobacco, wheat and so on