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目的研究清肺康肤合剂对激素依赖性皮炎豚鼠模型的作用。方法取健康豚鼠50只,雌雄各半,随机取10只豚鼠为空白对照组,另外40只应用0.05%丙酸氯倍他索酊剂制造豚鼠激素依赖性皮炎动物模型。造模成功后,分为模型组、清肺康肤合剂低剂量组、清肺康肤合剂高剂量组及阳性药物组,每组10只,对各组豚鼠采取相应的干预措施28天。观察实验前后各组豚鼠的皮损、病理改变、血清总IgE含量变化情况。结果与空白对照组比较,其余各组豚鼠的皮损明显,血清IgE升高,差异有统计学意义(P<0.05,P<0.01),说明造模成功。与模型组比较,清肺康肤合剂低剂量组、清肺康肤合剂高剂量组皮肤外观好转,血清总IgE水平降低,差异有统计学意义(P<0.05);与清肺康肤合剂低剂量组比较,清肺康肤合剂高剂量组血清IgE降低,差异有统计学意义(P<0.05)。病理常规切片HE染色后镜下观察,各组均未见明显变化。结论清肺康肤合剂对豚鼠激素依赖性皮炎有一定缓解作用。
Objective To study the effect of Qingfeikang Mixture on hormone-dependent dermatitis guinea pig model. Methods Fifty healthy guinea pigs were randomly divided into male and female. Ten guinea pigs were randomized into blank control group. Another 40 animals were treated with 0.05% clobetasol propionate tincture to make guinea pig hormone-dependent dermatitis animal model. After successful modeling, the rats were divided into model group, Qingfeikangfukao low dose group, Qingfeikangfukao high dose group and positive drug group, with 10 rats in each group. Guinea pigs in each group were given interventions for 28 days. Before and after the experiment observed guinea pigs lesions, pathological changes, changes in serum total IgE levels. Results Compared with the blank control group, the lesions of other guinea pigs were obvious and the serum IgE was increased, the difference was statistically significant (P <0.05, P <0.01), indicating successful modeling. Compared with the model group, the skin appearance of high-dose group of Qingfeikangfeiheji and Qingfeikangfukao agent improved, the serum total IgE level decreased, the difference was statistically significant (P <0.05) Compared with the dose group, the serum IgE of high-dose group of Qingfeikangfei Mixture was decreased, the difference was statistically significant (P <0.05). Histopathological sections were stained with HE and observed microscopically. There was no obvious change in each group. Conclusion Qingfeikangfu Mixture can relieve hormone-dependent dermatitis in guinea pigs.