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目的探讨胞嘧啶脱氨酶(CD)/5-氟胞嘧啶(5-FC)基因治疗联合放疗对裸鼠宫颈癌移植瘤的生长抑制作用,以及CD/5-FC基因治疗与放疗之间是否具有协同作用。方法将24只鼠龄6～8周成功接种了HeLa细胞的BALB/C雌性裸鼠,随机分为对照组、基因治疗组、放疗组以及联合治疗组,每组6只,观察各组治疗前后的肿瘤体积、肿瘤生长抑制率及肿瘤体积增加倍数。结果(1)治疗21d后,基因治疗组裸鼠的肿瘤体积是(728±201)mm3,联合治疗组是(357±113)mm3,放疗组是(739±419)mm3,对照组是(1168±380)mm3,基因治疗组、联合治疗组、放疗组肿瘤体积均小于对照组,3组分别与对照组比较,差异有统计学意义(P<0.05);联合治疗组肿瘤体积小于基因治疗组及放疗组,前者分别与后两组比较,差异有统计学意义(P<0.05)。(2)放疗组的肿瘤生长抑制率为36.74%,基因治疗组为37.66%,联合治疗组为69.45%,联合治疗组明显高于放疗组及基因治疗组,前者分别与后两组比较,差异有统计学意义(P<0.05)。(3)联合治疗组平均肿瘤体积增加倍数为7.71倍,基因治疗组为19.16倍,放疗组为18.86倍,对照组为40.57倍。析因设计的方差分析结果,CD/5-FC基因治疗与放疗之间有交互作用(P=0.03),当与放疗联合使用时,CD/5-FC基因治疗的疗效大于其单独使用时所产生的疗效;当与CD/5-FC基因治疗联合应用时,放疗的疗效大于其单独使用时所产生的疗效。结论CD/5-FC基因治疗与放疗联合应用,对裸鼠宫颈癌移植瘤的生长具有抑制作用,并且两者之间具有协同作用。
Objective To investigate the inhibitory effects of cytosine deaminase (CD) / 5-fluorocytosine (5-FC) gene therapy combined with radiotherapy on the growth of cervical cancer xenografts in nude mice and whether or not CD / 5-FC gene therapy and radiotherapy Has synergy. Methods Twenty-four BALB / C female nude mice, HeLa cells, were randomly divided into control group, gene therapy group, radiotherapy group and combination therapy group, with 6 mice in each group. Tumor volume, tumor growth inhibition rate and tumor volume increase multiple. Results (1) After 21 days of treatment, the tumor volume of gene therapy group was (728 ± 201) mm3, that of combination therapy group was (357 ± 113) mm3, that of radiotherapy group was (739 ± 419) mm3, and that of control group was ± 380) mm3. The tumor volume of gene therapy group, combination therapy group and radiotherapy group were less than that of control group, and there were significant differences between the three groups and the control group (P <0.05). The tumor volume of combination therapy group was smaller than that of gene therapy group And radiotherapy group, the former were compared with the latter two groups, the difference was statistically significant (P <0.05). (2) The tumor growth inhibition rate was 36.74% in radiotherapy group, 37.66% in gene therapy group and 69.45% in combination therapy group. The combination therapy group was significantly higher than radiotherapy group and gene therapy group, the former was respectively compared with the latter two groups There was statistical significance (P <0.05). (3) The mean tumor volume multiplication factor was 7.71 fold in the combination therapy group, 19.16 times in the gene therapy group, 18.86 times in the radiotherapy group and 40.57 times in the control group. Analysis of ANOVA resulted in an interaction between CD / 5-FC gene therapy and radiotherapy (P = 0.03). When combined with radiotherapy, the efficacy of CD / 5-FC gene therapy was greater than that of CD / 5-FC alone The efficacy of radiotherapy when combined with CD / 5-FC gene therapy is greater than the efficacy of radiotherapy alone. Conclusions The combination of CD / 5-FC gene therapy and radiotherapy can inhibit the growth of cervical cancer xenografts in nude mice and has a synergistic effect.