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Enzyme was immobilized on an ammonium ion-selective electrode by different methods.An ion-selective electrode is not completely ion-specific,and interfering ions react with the ion-selective electrode membrane,altering the measured potential.Therefore,the characteristics of the effect of other ions on ammonium ion-selective electrode-based urea biosensors are considered.Based on the experimental results,the urea biosensor based on entrapment had a high response voltage of around 189 mV and fast response time of around 16 sec.Moreover,selectivity of the urea biosensor in different interfering ions was considered to elucidate the characteristics of ammonium ion-selective electrode-based biosensors.
Enzyme was immobilized on an ammonium-selective electrode by different methods. Ion-selective electrode is not completely ion-specific, and interfering ions react with the ion-selective electrode membrane, altering the measured potential. Beforefore, the characteristics of the effect of other ions on ammonium ion-selective electrode-based urea biosensors are considered.Based on the experimental results, the urea biosensor based on entrapment had a high response voltage of around 189 mV and fast response time of around 16 sec. the urea biosensor in different interfering ions was considered to elucidate the characteristics of ammonium ion-selective electrode-based biosensors.