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目的探讨颅脑损伤患者血清与脑脊液IL-1β及NSE水平的变化及临床意义。方法选取2014-06—2016-01我院收治的96例颅脑损伤患者为研究对象(实验组),选取同期门诊健康者30例为对照组,应用双抗体夹心酶联免疫吸附试验法(ELISA)检测样本IL-1β及NSE的含量,比较实验组发病入院后不同时间点与对照组血清与脑脊液IL-1β及NSE的水平。依据患者入院时的格拉斯哥昏迷量表(GCS)评分将其分为轻度组(n=36)、中度组(n=32)和重度组(n=28),对比各组患者血清与脑脊液IL-1β及NSE水平的变化。结果观察组血清及脑脊液IL-1β及NSE水平均明显高于对照组(P<0.05);发病入院1d、3d,颅脑损伤患者血清及脑脊液IL-1β及NSE水平均呈升高趋势(P<0.05),且在发病后第3天达到高峰;发病入院后第7天,血清及脑脊液IL-1β及NSE水平均轻微下降。轻度、中度、重度组血清及脑脊液IL-1β及NSE水平均明显高于对照组,且随着病情程度的加重,血清及脑脊液IL-1β及NSE水平均呈升高趋势,血清及脑脊液IL-1β及NSE水平与患者病情程度具有明显相关性。脑脊液中IL-1β及NSE水平均高于血清(P<0.05)。结论颅脑损伤后患者血清及脑脊液IL-1β及NSE水平可反映颅脑损伤程度,可作为判断颅脑损伤患者预后的敏感指标。
Objective To investigate the changes and clinical significance of IL-1β and NSE in serum and cerebrospinal fluid (CSF) in patients with craniocerebral injury. Methods Ninety-six patients with craniocerebral injury treated in our hospital from June 2014 to June 2016 were selected as the experimental group. Thirty healthy subjects in the same period were selected as the control group. ELISA was performed using double antibody sandwich enzyme-linked immunosorbent assay (ELISA) ) Levels of IL-1β and NSE were measured. The levels of IL-1β and NSE in serum and cerebrospinal fluid of the experimental group at different time points after admission were compared. The patients were divided into mild group (n = 36), moderate group (n = 32) and severe group (n = 28) according to Glasgow Coma Scale (GCS) IL-1β and NSE levels. Results The levels of IL-1β and NSE in serum and cerebrospinal fluid of the observation group were significantly higher than those of the control group (P <0.05). The levels of IL-1β and NSE in serum and cerebrospinal fluid of patients with craniocerebral injury showed an increasing trend on the 1st and 3rd day <0.05), and reached its peak on the third day after onset. On the seventh day after admission, the levels of IL-1β and NSE in serum and CSF decreased slightly. The level of IL-1β and NSE in serum, cerebrospinal fluid and serum of mild, moderate and severe group were significantly higher than that of control group. The level of IL-1β and NSE in serum and cerebrospinal fluid increased with the severity of illness. Serum and cerebrospinal fluid The levels of IL-1β and NSE were significantly correlated with the severity of the patients. The levels of IL-1β and NSE in CSF were higher than those in serum (P <0.05). Conclusion The level of IL-1β and NSE in serum and cerebrospinal fluid of patients with craniocerebral injury can reflect the degree of brain injury and can be used as a sensitive index to judge the prognosis of patients with craniocerebral injury.