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抗结核药物的复合制剂有其突出的优点。其含量的测定繁杂费时，需要逐个药物分别测定。本文报告一种可以同时测定异烟肼和利福平两种药物的高效液相色谱法。本法用Ｓｐｈｅｒｉｓｏｒｂ Ｃ８（４．６×２５０ｍｍ，５μｍ，ＩＤ）色谱柱，以甲醇：０．０５ＭＮａＡｃ／ＨＡＣ缓冲液（ｐＨ６．９０）＝６５：３５（Ｖ：Ｖ）为流动相，流速１．０ｍｌ／ｍｉｎ，紫外检测器检测（＝２５４ｎｍ）。结果：异烟肼和利福平的保留时间分别为３．０１１ｍｉｎ和６．７９ｍｉｎ，在１．０～２０．０μｇ／ｍｌ浓度范围内线性关系分别为：Ｙ＝２０１７．４０Ｘ－３３９．９５，ｒ＝０．９９９７；Ｙ＝２１００．７０Ｘ＋１２５．７６，ｒ＝０．９９９５，日内变异分别为 １．８３％、２．０８％；日间差异分别为 １．８９％、２．１７％。回收率分别为 ９９．７２％、９８．２７％。本法快速，可同时测定两种药物。
The anti-TB drug combination has its outstanding advantages. The determination of its content is cumbersome and time-consuming, and it is necessary to measure the drug individually. This article reports a high performance liquid chromatographic method that can simultaneously determine both isoniazid and rifampicin. This method uses a Spherisorb C8 (4.6 × 250 mm, 5 μm, ID) column with methanol: 0.05 M NaCl / HAC buffer (pH 6.90) = 65:35 (V: V) as the mobile phase at a flow rate of 1. 0ml / min, UV detector detection (= 254nm). RESULT: The retention times of isoniazid and rifampicin were 3.011min and 6.79min, respectively. The linear relationship of isoniazid and rifampicin at the concentration range of 1.0 ~ 20.0μg / ml were Y = 2017. 40X-339.95, r = 0.9997; Y = 2100.70X + 125.76, r = 0.9995, the intra-day variation was 1.83% and 2.08% respectively; the daytime differences were 1.89% and 2.17% respectively. The recoveries were 99.72% and 98.27% respectively. This method is fast and can measure two drugs at the same time.