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目的：探讨抗ＰＭＬ－ＲＡＲα反义核酸对早幼粒白血病细胞生长、细胞周期、细胞凋亡的作用。方法：以ＮＢ４细胞株为研究对象；细胞计数采用台盼兰排除、计数板计数法；白血病细胞集落采用甲基纤维素半固体培养；流式细胞仪（ＦＣＭ）用于检测细胞凋亡和细胞周期。结果：以６０μｇ／ｍｌ的终浓度处理细胞，抗 ＰＭＬ－ＲＡＲα融合部位反义核酸（ＦＵＡＳ）能明显抑制ＮＢ４细胞增殖及白血病细胞集落（ＡＭＬ－ＣＦＵ）形成，最大抑制率分别为４６．８％，５１．６％；ＧＭ－ＣＳＦ能减弱ＦＵＡＳ的作用，ＦＵＡＳ作用时间越短，ＧＭ－ＣＳＦ促ＡＭＬ－ＣＦＵ增加越明显；ＦＵＡＳ处理第７，９天，出现明显的凋亡细胞群，凋亡细胞百分比分别为４１．０％，３４．２％；ＦＵＡＳ处理第５天Ｓ期下降，Ｇ２／Ｍ期升高，第７天Ｃ０／Ｇ１期明显升高，Ｓ期回升。结论：抗ＰＭＬ－ＲＡＲα反义核酸具有抑制早幼粒白血病细胞增殖、诱导细胞凋亡的作用。
Objective: To investigate the effect of anti-PML-RARα antisense nucleic acid on the growth, cell cycle and apoptosis of human promyelocytic leukemia cells. Methods: The NB4 cell line was used as the research object. The cell count was determined by trypan blue exclusion and counting plate counting. The leukemic cell colonies were cultured with methylcellulose semi-solid. Flow cytometry (FCM) cycle. Results: The cells treated with anti-PML-RARα antisense oligonucleotide (FUAS) at a final concentration of 60μg / ml significantly inhibited the proliferation of NB4 cells and the formation of leukemic cell colonies (AML-CFU) with the maximum inhibition rates of 46.8% , 51.6% respectively. GM-CSF attenuated the effect of FUAS. The shorter the time of FUAS was, the more obvious the increase of AML-CFU was induced by GM-CSF. On the 7th and 9th day of FUAS treatment, The percentages of cells were 41.0% and 34.2%, respectively. On the fifth day after FUAS treatment, the S phase decreased and the G2 / M phase increased. On the seventh day, the C0 / G1 phase increased significantly and the S increased. Conclusion: The anti-PML-RARα antisense oligonucleotide can inhibit the proliferation and induce the apoptosis of human promyelocytic leukemia cells.