论文部分内容阅读
利用富集驯化培养、稀释涂布的方法,从连作黄瓜根际土壤中分离得到1株能降解肉桂酸的真菌2-4。该菌能够在以肉桂酸为唯一碳源的无机盐培养基上生长;经形态学、生理生化及分子等分类方法鉴定其为黑曲霉(Aspergillus niger)。该菌不同培养时间的肉桂酸降解液对黄瓜种子发芽试验表明,该菌可有效降解肉桂酸,从而缓解对黄瓜发芽的抑制作用,72 h降解液处理的种子发芽率、发芽指数和活力指数可分别达到对照的100%、92.06%和91.49%;胚根长、根表面积和根尖数达到对照的84.19%、87.55%和82.64%,而胚轴长、鲜质量差异不显著。
Using enrichment and acclimation cultivation, dilution coating method, from the continuous cropping rhizosphere soil isolated a strain of cinnamic acid-degrading fungi 2-4. The strain could grow on inorganic salt medium with cinnamic acid as the sole carbon source. It was identified as Aspergillus niger by morphological, physiological, biochemical and molecular classification methods. The germination test of cinnamic acid degradation solution of cucumber at different culture time showed that the bacteria could effectively degrade cinnamic acid and alleviate the inhibition of cucumber seed germination. The seed germination rate, germination index and vigor index Reaching 100%, 92.06% and 91.49% of the control respectively. The root length, root surface area and root tip number reached 84.19%, 87.55% and 82.64% of the control, respectively.