Background. We attempted to determine the prevalence of genital human papillomavirus (HPV) infection in women attending gynecologic practitioners in South Taiwan. Methods. The population included 4383 women aged 16- 78 seeking HPV testing at primary gynecologic practitioners regardless of their cervical cytology results. HPV DNA was identified from cervical swabs using semi- nested polymerase chain reaction with MY11, MY09/HMB01, and MY11/bioGP6+ primers. Genotyping for high- risk HPV (HR- HPV) was done separately by a HR- HPV chip, which contained 13 type- specific oligonu- cleotides on a nylon membrane. Results. The overall HPV prevalence was 19.3% (849/4383), 11.1% (488/4383) were confirmed as HR- HPV positive. Among the women with HR- HPV infection, HPV- 16 was the most prevalent type (22.1% ; 108/488), followed by HPV- 52 (21.3% ; 104/488), and HPV- 58 (19.9% ; 97/488). Multiple infections were detected in 73 women (15.0% ; 73/488). For women with age 30 or younger, the overall HPV and HR- HPV prevalence were 32.0% and 20.7% , respectively, which were significantly higher than those of women age older than 30 (17.2% and 9.5% , P < 0.001). More multiple infections (22.1% vs. 12.4% ) were also found in women with age 30 or younger (P = 0.021). However, the relative contribution of types to the overall HR- HPV positive among different age groups remains the same. Conclusions. Our results showed an HPV prevalence that is similar compared with worldwide levels. HPV prevalence and multiple infections rate were decreasing across the age groups. Unlike most previous studies, the relative high prevalence of HPV 52 and 58 among South Taiwan women has important implications in vaccine prophylaxis. Background. We attempted to determine the prevalence of genital human papillomavirus (HPV) infection in women attending gynecologic practitioners in South Taiwan. Methods. The population included 4383 women aged 16- 78 seeking HPV testing at primary gynecologic practitioners regardless of their cervical cytology results. HPV DNA was identified from cervical swabs using semi-nested polymerase chain reaction with MY11, MY09 / HMB01, and MY11 / bioGP6 + primers. Genotyping for high- risk HPV (HR- HPV) was done separately by a HR- HPV chip, which contained Results The The HPV prevalence was 19.3% (849/4383), 11.1% (488/4383) were confirmed as HR-HPV positive. Among the women with HR-HPV infection , HPV-16 was the most prevalent type (22.1%; 108/488), followed by HPV- 52 (21.3%; 104/488), and HPV- 58 73 women (15.0%; 73/488). For women with age 30 or younger, the overall HP V and HR-HPV prevalence were 32.0% and 20.7%, respectively, which were significantly higher than those of women older than 30 (17.2% and 9.5%, P <0.001). More multiple infections (22.1% vs. 12.4% However, the relative contribution of types to the overall HR- HPV positive among different age groups remains the same. Conclusions. Our results showed an HPV prevalence that is similar with worldwide levels. HPV most prevalence and multiple infections rate were decreased across the age groups. Unlike most previous studies, the relative high prevalence of HPV 52 and 58 among South Taiwan women has important implications in vaccine prophylaxis.