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目的利用静电纺丝技术制备丹参素-聚己内酯(DSS-PCL)纳米纤维支架。方法以二氯甲烷-N,N-二甲基甲酰胺(CH2Cl2-DMF)8∶2为溶剂,利用静电纺丝仪制备载丹参素的纳米纤维,通过扫描电镜(SEM)观察纤维形貌,通过体外释放实验考察载药纳米纤维支架中丹参素的释放规律,通过MTT法和溶血率对载药纳米纤维支架的安全性进行初步评价。结果 CH2Cl2-DMF混合溶剂体系下药物和材料都能够很好地溶解并顺利电纺得到纳米纤维,平均纤维直径分别为210 nm和190 nm,体外释放实验表明药物的释放经过一个快速释放后进入缓慢释放。MTT法测得PCL及载有丹参素的纳米纤维对L929细胞存活率并无明显影响,溶血率实验测得各组纳米纤维的溶血率低于5%。结论静电纺丝法制备DSS-PCL纳米纤维的工艺简单易行,易于推广。
Objective To prepare Danshensu-polycaprolactone (DSS-PCL) nanofiber scaffolds by electrospinning. Methods Danshensu nanofibers were prepared by electrospinning with dichloromethane-N, N-dimethylformamide (CH2Cl2-DMF) 8:2. The morphology of the fibers was observed by scanning electron microscopy (SEM) The release of Danshensu in drug-loaded nanofiber scaffolds was investigated by in vitro release assay. The safety of drug-loaded nanofibrous scaffolds was evaluated by MTT assay and hemolysis rate. Results The drugs and materials were well dissolved and electrospun into nanofibers under the condition of CH2Cl2-DMF. The average fiber diameters were 210 nm and 190 nm, respectively. In vitro release experiments showed that the release of the drug slowed down after a quick release freed. MTT assay measured PCL and nano-fibers containing Danshensu L929 cell survival rate had no significant effect, hemolysis rate measured in each group of nano-fiber hemolysis rate of less than 5%. Conclusion Electrospinning DSS-PCL nanofibers technology is simple and easy to promote.