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目的建立快速、准确的 HL A基因分型方法 ,满足临床移植配型的需要。方法通过序列特异性引物聚合酶链反应 (PCR- SSP) ,对拟行骨髓移植的 10例血液病患者及 12名相关供者的 HL A- 类基因 DRB1,DQB1位点扩增 ,琼脂糖凝胶电泳分析 PCR产物并确定其基因型。结果发现 2例患者与其相关供者 HL A- DRB1,DQB1基因全相同 ,其中 1例成功进行了骨髓移植。结论该方法具有快速准确、特异性高、简便省时的特点。
Objective To establish a rapid and accurate method of HLA genotyping to meet the needs of clinical transplantation matching. Methods The HLA-A DRB1 and DQB1 loci were amplified by PCR-SSP in 10 hematologic patients and 12 related donors. Agarose gel The PCR product was analyzed by gel electrophoresis and its genotype was determined. The results showed that HL-DRB1 and DQB1 genes were identical in 2 patients and their related donors, of which 1 patient successfully underwent bone marrow transplantation. Conclusion The method has the characteristics of fast and accurate, high specificity, simple and time-saving.