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苹果褐斑病菌(Marssonina coronaria(Ell·et Davis) Davis)以芽管或附着胞一侵染丝直接穿透表皮侵入,叶背与叶面的侵入比为7:3。分生孢子萌发与营养条件关系密切,在蒸馏水中萌发率低,1％葡萄糖、10％苹叶浸汁及1％琼脂有促进作用,尤以琼脂的效果显著;分生孢子荫发适温为18～25℃,最适pH为6～7;水滴为孢子萌发所必须;光照的影响不大。该病菌适合用单孢分离。在PDA培养基上培养,菌丝不发达,但能产生分生孢子盘及分生孢子。
The pathogen invaders (Marssonina coronaria (Ell. Et Davis) Davis) penetrate the epidermis directly through germ tube or attachment of infected cells. The invade ratio of leaves to leaves is 7: 3. Conidia germination and nutrition conditions are closely related to the low germination rate in distilled water, 1% glucose, 10% apple juice and 1% agar agar, especially in agar effect was significant; conidia shade suitable temperature 18 ~ 25 ℃, the optimum pH is 6 ~ 7; water droplet is necessary for spore germination; light has little effect. The bacteria suitable for single spore separation. In PDA medium culture, mycelium underdeveloped, but can produce conidia disk and conidia.