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以茶树菇为试材,采用液体摇瓶培养法,通过单因素试验、正交实验,以菌丝体生物量为主要指标,对茶树菇液体发酵培养基配方及培养条件进行优化。结果表明:最适宜的培养基配方为葡萄糖3.0%、豆饼粉1.0%、酵母膏0.2%、MgSO_4·7H_2O 0.10%、KH_2PO_40.10%。适宜的茶树菇液体培养条件为起始pH 6.0~7.0、培养温度25~28℃、摇床转速150~180r·min~(-1)。
Using Agrocybe aegerus as test material, liquid shake flask culture method was adopted to optimize the formula and culture conditions of Agrocybe aegerita liquid fermentation medium by single factor experiment and orthogonal experiment with mycelial biomass as the main index. The results showed that the most suitable medium was glucose 3.0%, soybean meal 1.0%, yeast extract 0.2%, MgSO 4 4.7H 2 O 0.10% and KH 2 PO 40.10%. The optimum conditions for the liquid culture of A. sinensis were pH 6.0-7.0, temperature 25- 28 ℃ and shaking speed 150-180 r · min -1.